台灣大學漁業科學研究所

研究重點：
一、抗菌肽
(1) 抗菌肽在水產養殖上之研發與應用
(2) 抗菌肽在敗血症上之基礎研究與應用
(3) 抗菌肽在癌症上之基礎研究與應用
 
二、基因轉殖技術
(1) 螢光觀賞魚之研發
(2) 螢光觀賞蝦之研發

目前進行的研究計畫：
1. 探討Epinecidin-1在抗菌活性以外對於敗血性關節炎之治療效果 (科技部)
2. Improvement of Omega-3 Fatty Acids Biosynthesis in Tilapia for Cold Adaption and Immune Modulation by Omics Approaches to Advance Aquaculture Industry (中研院)
3. Application of NGS for Genomic Industry in Agriculture – Molecular Assisted Breeding on Giant Grouper (農委會)
4. The application of antimicrobial peptides as feed additives for fowls (農委會)

抗菌胜肽，就是一種可以對抗微生物的胜肽，小於100個胺基酸的一種胜肽，又名「抗菌蛋白」。抗菌胜肽幾乎可以在各種生物中被發現，而抗菌胜肽更是生物個體先天性免疫中重要的一環。抗菌胜肽都有一些共通的特性: 分子量小、帶正電(帶有+2~+9價電荷)、具有明顯的兩極性，因此抗菌胜肽才能與磷脂膜有很強的相互作用與滲透磷脂膜的能力，這也是抗菌胜肽之所以具有抗菌功能的關鍵；但是在眾多抗菌胜肽上，不論在立體結構或是胺基酸序列上，都有許多差異性。近年來研究發現，抗菌胜肽除了能對抗革蘭氏陰性菌與革蘭氏陽性菌之外，更多的研究學者發現，抗菌胜肽還具有對抗臨床致病菌株、黴菌、真菌、寄生蟲與具有外套膜的病毒，更還發現具有對抗癌症的功能，因此具有抗癌功能的此類抗菌胜肽則特別稱為抗癌胜肽，這對急於發展新興的抗癌藥物而言，是一項很重大發現。實驗室的研究方向與興趣如下：

1) 使用各種修飾方法，例如蛋白質工程學、化學工程學，修改抗菌肽並降低其毒性、溶血性，增強其在不同離子濃度與血液中之半衰期長度。

2) 探討抗菌肽在抗革蘭氏陰性菌、抗革蘭氏陽性菌、抗黴菌、抗真菌、抗寄生蟲、抗病毒，與抗癌症上的機能性與相關藥物研發。

3) 抗菌胜肽在細胞上之分子機制探討。

近年重要著作 (2017- ):

*責任作者

Hsueh-Ming Tai, Ming-Feng You, Chia-Hua Lin, Tsung-Yu Tsai, Chieh-Yu Pan#, Jyh-Yih Chen* (2021). Scale-up production of and dietary  supplementation with the recombinant antimicrobial peptide tilapia piscidin 4 to improve growth performance in Gallus gallus domesticus. Plos one (in press) *Corresponding author.

Pin-Yang Tu, Shin-Jie Huang, Venugopal Rajanbabu, Jen-Leih Wu, Jyh-Yih Chen* (2021). Comparative transcriptome analysis reveals ectopic delta-5 and delta-6 desaturases enhance protective gene expression upon Vibrio vulnificus challenge in Tilapia (Oreochromis niloticusis). BMC genomics (in press) *Corresponding author.

Bor-Chyuan Su, Giun-Yi Hung, Yun-Chieh Tu, Wei-Chen Yeh, Meng-Chieh Lin, Jyh-Yih Chen* (2021). Marine antimicrobial peptide TP4 exerts anticancer effects on human synovial sarcoma cells via calcium overload, reactive oxygen species production and mitochondrial hyperpolarization. Marine drugs (in press) *Corresponding author.

Bor-Chyuan Su, Chao-Chin Li, Chia-Wen Liu, Jyh-Yih Chen* (2020). A pilot safety assessment for recombinant Epinephelus lanceolatus piscidin yeast powder as a drug food additive after subacute and subchronic administration to SD rats. Marine drugs (in press) *Corresponding author.

Bor-Chyuan Su, Yi-Chung Liu, Chen-Hung Ting, Ping-Chiang Ly, Jyh-Yih Chen* (2020). Antimicrobial peptide TP4 targets mitochondrial adenine nucleotide translocator 2. Marine drugs (in press) *Corresponding author.

Bor-Chyuan Su, Chen-Hung Ting, Kang-Yun Lee, Sheng-Ming Wu, Po-Hao Feng, Yao-Fei Chan, Jyh-Yih Chen* (2020). Novel PD-L1 mAb HC16 reveals upregulation of PD-L1 in BAC subtype and early-stage. Histology and Histopathology (in press) *Corresponding author.

Keng-Yu Chiang, Wen-Chun Lin, Tsung-Yu Tsai, Cheng-Wei Lin, Shin-Jie Huang, Ching-Yu Huang, Sheng-Han Wu, Chuian-Fu Ken, Hong-Yi Gong*, Jyh-Yih Chen*, Jen-Leih Wu* (2020). Dual expression of transgenic delta-5 and delta-6 desaturase in tilapia alters gut microbiota and enhances resistance to Vibrio vulnificus infection. PLOS ONE (in press) *Corresponding author.

Prakash Kishore Hazam, Jyh-Yih Chen* (2020). Therapeutic utility of the antimicrobial peptide Tilapia Piscidin 4 (TP4). Aquaculture reports (in press) *Corresponding author.

Bor-Chyuan Su, Chao-Chin Li, Jiun-Lin Horng, Jyh-Yih Chen* (2020). Calcium-dependent calpain activation-mediated mitochondrial dysfunction and oxidative stress are required for cytotoxicity of epinecidin-1 in human synovial sarcoma SW982 cells. International Journal of Molecular Sciences (in press) *Corresponding author.

Hsiao-Ching Chen, Chieh-Yu Pan, Venugopal Rajanbabu, Yen-Yun Lee, Wei-Ren Tsai, Jyh-Yih Chen* (2020). Lack of acute toxicity and mutagenicity from recombinant Epinephelus lanceolatus piscidin expressed in Pichia pastoris. Marine drugs (in press) *Corresponding author.

Hsueh-Ming Tai, Han-Ning Huang, Tsung-Yu Tsai, Ming-Feng You, Hung-Yi Wu, Venugopal Rajanbabu, Hsiao-Yun Chang, Chieh-Yu Pan, Jyh-Yih Chen* (2020). Dietary supplementation of recombinant antimicrobial peptide Epinephelus lanceolatus piscidin improves growth performance and immune response in Gallus gallus domesticus. PLOS ONE (in press). *Corresponding author.

Bor-Chyuan Su, Jyh-Yih Chen* (2020). Pharmacological inhibition of p38 potentiates antimicrobial peptide TP4-induced cell death in glioblastoma cells. Molecular and Cellular Biochemistry 464(1-2):1-9. *Corresponding author.

Bor-Chyuan Su, Tsung-Han Wu, Chun-Hua Hsu, Jyh-Yih Chen* (2020). Distribution of positively charged amino acid residues in antimicrobial peptide epinecidin-1 is crucial for in vitro glioblastoma cytotoxicity and its underlying mechanisms. Chemico-Biological Interactions 315:108904. *Corresponding author.

Bor-Chyuan Su, Jyh-Yih Chen* (2020). Epinecidin-1: an orange-spotted grouper antimicrobial peptide that modulates Staphylococcus aureus lipoteichoic acid-induced inflammation in macrophage cells. Fish & Shellfish Immunology 99:362-367. *Corresponding author.

Han-Ning Huang, Tsung-Yu Tsai, Venugopal Rajanbabu, Chieh-Yu Pan, Jyh-Yih Chen* (2020). Dietary supplementation of recombinant Tilapia Piscidin-4-expressing yeast enhances growth and immune response in Lates calcarifer. Aquaculture Reports (in press) *Corresponding author.

Bor-Chyuan Su, Chieh-Yu Pan, Jyh-Yih Chen* (2019). Antimicrobial peptide TP4 induces ROS-mediated necrosis by triggering mitochondrial dysfunction in wild-type and mutant p53 glioblastoma cells. Cancers (Basel). Cancers (Basel). 2019 Feb 1;11(2). pii: E171. doi: 10.3390/cancers11020171. *Corresponding author.

Han-Ning Huang, Jyh-Yih Chen* (2018). Evaluation of antimicrobial peptides as antiviral agents against foot and mouth disease virus. Peptides (in press) *Corresponding author.

Bor-Chyuan Su, Wen-Chun Lin, Jyh-Yih Chen* (2018). Recombinant Epinephelus lanceolatus serum amyloid A as a feed additive: Effects on immune gene expression and resistance to Vibrio alginolyticus infection in Epinephelus lanceolatus. Fish and Shellfish Immunology (in press) *Corresponding author.

Bor-Chyuan Su, Yung-Wei Lai, Jyh-Yih Chen*, Chieh-Yu Pan* (2018). Transgenic expression of tilapia piscidin 3 (TP3) in zebrafish confers resistance to Streptococcus agalactiae. Fish and Shellfish Immunology (in press) *Corresponding author.

Bor-Chyuan Su, Jyh-Yih Chen* (2017). Antimicrobial peptide epinecidin-1 modulates MyD88 protein expression via the proteasome degradation pathway. Marine drugs (in press) *Corresponding author.

Bor-Chyuan Su, Han-Ning Huang, Tai-Wen Lin, Chwan-Deng Hsiao, Jyh-Yih Chen* (2017). Epinecidin-1 protects mice from LPS-induced endotoxemia and cecal ligation and puncture-induced polymicrobial sepsis. BBA - Molecular Basis of Disease (in press) *Corresponding author.

Chuian-Fu Ken, Chieh-Ning Chen, Chen-Hung Ting, Chieh-Yu Pan*, Jyh-Yih Chen* (2017). Transcriptome analysis of hybrid tilapia (Oreochromis spp.) with Streptococcus agalactiae infection identifies Toll-like receptor pathway-mediated induction of NADPH oxidase complex and piscidins as primary immune-related responses. Fish and Shellfish Immunology (in press) *Corresponding author.

Bor-Chyuan Su, Wen-Chun Lin, Han-Ning Huang, Jyh-Yih Chen* (2017). Recombinant expression of Epinephelus lanceolatus serum amyloid A (ElSAA) and analysis of its macrophage modulatory activities. Fish and Shellfish Immunology 64:276-286. *Corresponding author.

Chieh-Yu Pan, Yu-Hao Liu, Hong-Yi Gong, Jyh-Yih Chen* (2017). Transcriptome analysis of the effect of polyunsaturated fatty acids against Vibrio vulnificus infection in Oreochromis niloticus. Fish and Shellfish Immunology 62:153-163. *Corresponding author.

Han-Ning Huang, Chieh-Yu Pan, Hung-Yi Wu*, Jyh-Yih Chen* (2017). Antimicrobial peptide Epinecidin-1 promotes complete skin regeneration of methicillin-resistant Staphylococcus aureus-infected burn wounds in a swine model. Oncotarget 8(13):21067-21080. *Corresponding author.

Chieh-Yu Pan*, Tsung-Yu Tsai, Bor-Chyuan Su, Cho-Fat Hui, Jyh-Yih Chen* (2017). Study of the antimicrobial activity of tilapia piscidin 3 (TP3) and TP4 and their effects on immune functions in hybrid tilapia (Oreochromis spp.). Plos one 12(1):e0169678. *Corresponding author.