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NTUBST2019

專任教師

張世宗 教授
連絡資訊


張世宗 教授

張世宗 (Shih-Chung Chang) 教授

國立臺灣大學 生命科學院 生化科技學系

  • 電話 :(O) 3366-3740 ;(L) 3366-4441
  • 傳真 :3366-2271
  • 辦公室 :農化二館 508 室
  • 研究室 :細胞生物學研究室 (農化二館 515 室)
  • E-mail :shihchung@ntu.edu.tw
  • 研究專長 :細胞生物學、分子生物學、生物化學
  • 授課領域 :生物化學、生物化學實驗、酵素純化與分析及實驗、生化科技實驗法、單株抗體、免疫學技術-抗體工具、生物技術核心實驗

學歷


  • 國立台灣大學 農業化學所 博士 (1995/09 ~ 1999/10)
  • 國立台灣大學 農業化學所 碩士 (1994/09 ~ 1995/08)
  • 國立台灣大學 農業化學系 學士 (1990/09 ~ 1994/06)
  • 台北市立建國中學 (1987/09 ~ 1990/06)

研究焦點


類泛素蛋白質 (ubiquitin-like proteins) 修飾系統:

研究室在ubiquitin-proteasome及small ubiquitin-like proteins等蛋白質後轉譯修飾系統的研究方面,著重於探討26S proteasome之19S regulatory particle ATPase (Rpt) 之SUMO化修飾、ubiquitin-specific protease (USP)與NEDD8-specific protease (SENP8) 之基質專一性辨識及作用機制。我們已經鑑定出Rpt分子上被SUMO修飾的胺基酸位置,並且證實Rpt被SUMO修飾後會改變其ATPase活性與在細胞內之位置,亦會影響proteasome之酵素活性;USP2主要是透過辨識ubiquitin與NEDD8之4、12、14與72號之胺基酸序列的保守性不同,而SENP8是透過辨識ubiquitin與NEDD8之51與72號之胺基酸序列的保守性不同,來達到專一性辨識及區別ubiquitin及NEDD8的作用機制,相關研究成果已經發表於PLoS One與Scientific Reports期刊。

 

免疫學技術之創新性應用:

研究室長期投入免疫學技術之創新性研究與應用,目前已成功完成薄膜塗佈式西方墨點分析法之研發,經進一步改良後,再搭配真空吸引及毛細管塗佈技術,使抗體用量可減少至傳統方法之百分之一,顯著地降低高單價之抗體的用量,並且能於10分鐘之內完成原本需要三小時才能完成之西方墨點分析實驗,未來將可應用於大量的抗原及抗體篩選實驗,此新穎之薄膜塗佈式西方墨點分析法已發表兩篇研究論文於Analytical Chemistry期刊中,並已取得美國及本國之專利。

 

新興感染症之相關研究與診斷試劑研發:

研究室亦積極投入新興感染症之相關研究工作,尤其著重在新型H7N9流感病毒、H5N3/H5N6/H5N8禽流感病毒、茲卡病毒 (Zika virus)、中東呼吸道症候群冠狀病毒 (MERS-CoV) 及2019新型冠狀病毒 (SARS-CoV-2) 之重組蛋白質表現純化與單株抗體的製備。針對上述之多種病毒,我們已成功研發出一系列具有高專一性及病毒中和力的單株抗體,並且皆已運用於快篩技術及分子檢測平台之開發,相關研究成果已發表於國際期刊及進行技轉授權,希望對新興感染症的研究、診斷與治療領域能有所貢獻。

 

 

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主要經歷


  • 生化科技學系 教授 (2017/08 ~ now)
  • 生化科技學系 副教授 (2012/08 ~ 2017/07)
  • 生化科技學系 助理教授 (2006/08 ~ 2012/07)
  • 美國哈佛大學醫學院細胞生物學系 博士後研究員 (2001/08 ~ 2005/07)

榮譽獎項


  • 臺灣大學教學傑出獎 (2019)
  • 臺灣大學教學優良獎 (2018)
  • 臺灣大學教學優良獎 (2017)
  • 臺灣大學教學優良獎 (2011)

 

代表著作


  1. Hsiao CC, Chiang YW, Chao TL, Tsai ZU, Wang TX, Jiang YW, Hsu HF, Lu DC, Wang JT, Wang JR*, Wang AB*, Chang SY*, and Chang SC*. Simultaneous detection of antibody responses to multiple SARS-CoV-2 antigens by a Western blot serological assay. Appl Microbiol Biotechnol. 2022 Nov 21. doi: 10.1007/s00253-022-12288-0.(*Corresponding author) (SCI)
  2. Lai GC, Chao TC, Lin SY, Kao HC, Tsai YM, Lu DC, Chiang YW, Chang SY*, Chang SC*. Neutralization or enhancement of SARS-CoV-2 infection by a monoclonal antibody targeting a specific epitope in the spike receptor-binding domain. Antiviral Res. 2022; 200:105290. (*Corresponding author) (SCI)
  3. Li CJ, Chao TL, Chang TY, Hsiao CC, Lu DC, Chiang YW, Lai GC, Tsai YM, Fang JT, Ieong S, Wang JT, Chang SY*, Chang SC*. Neutralizing monoclonal antibodies inhibit SARS-CoV-2 infection through blocking membrane fusion. Microbiol Spectr. 2022; e0181421. (*Corresponding author) (SCI)
  4. Li CJ, Huang PH, Chen HW, Chang SC* (2021) Development and characterization of mouse monoclonal antibodies targeting to distinct epitopes of Zika virus envelope protein for specific detection of Zika virus. Appl Microbiol Biotechnol. 105(11):4663-4673. (*Corresponding author) (SCI)
  5. Chiang YW, Li CJ, Su HY, Hsieh KT, Weng CW, Chen HW, Chang SC*. Development of mouse monoclonal antibody for detecting hemagglutinin of avian influenza A(H7N9) virus and preventing virus infection. Appl Microbiol Biotechnol. 2021; 105(8):3235-3248. (*Corresponding author) (SCI)
  6. Cheng YC, Chang SC*. Development and biochemical characterization of the monoclonal antibodies for specific detection of the emerging H5N8 and H5Nx avian influenza virus hemagglutinins. Appl Microbiol Biotechnol. 2021; 105(1):235-245. (*Corresponding author) (SCI)
  7. Shin YC, Chen JH, Chang SC*. The molecular determinants for distinguishing between ubiquitin and NEDD8 by USP2. Scientific Reports. 2017; 7: 2304. (SCI)
  8. Lin YC, Chang SC*, Juang RH*. Plastidial alpha-glucan phosphorylase 1 complexes with disproportionating enzyme 1 in Ipomoea batatas storage roots for elevating malto-oligosaccharide metabolism. PLoS ONE. 2017; 12(5): e0177115.(SCI)
  9. Hu, CJ, Chien, CY, Liu, MT, Fang, ZS, Chang, SY, Juang, RH, Chang, SC, Chen, HW. Multi-antigen avian influenza a (H7N9) virus-like particles: particulate characterizations and immunogenicity evaluation in murine and avian models. BMC Biotechnol 2017; 17(1): 2.(SCI)
  10. Liu CY, Lu DC, Jiang YW, Yen YK, Chang SC*, and Wang AB* (2016). Easy and fast western blotting by thin-film direct coating with suction. Anal Chem. Jun 21;88(12):6349-56. (SCI).
  11. He JL, Chiu YC, Chang SC, Wang CH, Juang RH. Glycosylation at hemagglutinin Asn-167 protects the H6N1 avian influenza virus from tryptic cleavage at Arg-201 and maintains the viral infectivity. Virus Res 2015; 197: 101-7.(SCI)
  12. Young GH, Huang TM, Wu CH, Lai CF, Hou CC, Peng KY, Liang CJ, Lin SL, Chang SC, Tsai PR, Wu KD, Wu VC, Ko WJ. Hemojuvelin modulates iron stress during acute kidney injury: improved by furin inhibitor. Antioxid Redox Signal 2014; 20(8): 1181-94.(SCI)
  13. Yen YK, Jiang YW, Chang SC, Wang AB. Western blotting by thin-film direct coating. Anal Chem 2014; 86(10): 5164-70.(SCI)
  14. Huang TF, Cho CY, Cheng YT, Huang JW, Wu YZ, Yeh AY, Nishiwaki K, Chang SC, Wu YC. BLMP-1/Blimp-1 regulates the spatiotemporal cell migration pattern in C. elegansPLoS Genet 2014; 10(6): e1004428.(SCI)
  15. Sun CY, Chang SC, Wu MS. Uremic toxins induce kidney fibrosis by activating intrarenal renin-angiotensin-aldosterone system associated epithelial-to-mesenchymal transition. PLoS One. 2012; 7(3): e34026. (SCI)
  16. Sun CY, Chang SC, Wu MS. Suppression of Klotho expression by protein-bound uremic toxins is associated with increased DNA methyltransferase expression and DNA hypermethylation. Kidney Int.  2012; 81(7): 640-50. (SCI)
  17. Lin YC, Chen HM, Chou IM, Chen AN, Chen CP, Young GH, Lin CT, Cheng CH, Chang SC, Juang RH. Plastidial starch phosphorylase in sweet potato roots is proteolytically modified by protein-protein interaction with the 20S proteasome. PLoS One. 2012; 7(4): e35336. (SCI)
  18. Shin YC, Tang SJ, Chen JH, Liao PH, Chang SC. The molecular determinants of NEDD8 specific recognition by human SENP8. PLoS One. 2011; 6(11): e27742.(SCI)
  19. Nguyen TT, Chang SC, Evnouchidou I, York IA, Zikos C, Rock KL, Goldberg AL, Stratikos E, Stern LJ. Structural basis for antigenic peptide precursor processing by the endoplasmic reticulum aminopeptidase ERAP1. Nat Struct Mol Biol. 2011; 18(5): 604-13. (SCI)
  20. Liu LR, Lin SP, Chen CC, Chen YJ, Tai CC, Chang SC, Juang RH, Tseng YW, Liu BH, Mersmann HJ, Shen TL, Ding ST. Serum Amyloid A Induces Lipolysis by Downregulating Perilipin Through ERK1/2 and PKA Signaling Pathways. Obesity (Silver Spring). 2011. (SCI)
  21. Shin YC, Liu BY, Tsai JY, Wu JT, Chang LK, Chang SC. Biochemical characterization of the small ubiquitin-like modifiers of Chlamydomonas reinhardtii. Planta. 2010; 232(3): 649-62. (SCI)
  22. Rabl J, Smith DM, Yu Y, Chang SC, Goldberg AL, Cheng Y. Mechanism of gate opening in the 20S proteasome by the proteasomal ATPases. Mol Cell. 2008; 30(3): 360-8. (SCI)
  23. Evnouchidou I, Momburg F, Papakyriakou A, Chroni A, Leondiadis L, Chang SC, Goldberg AL, Stratikos E. The internal sequence of the peptide-substrate determines its N-terminus trimming by ERAP1. PLoS One. 2008; 3(11): e3658. (SCI)
  24. Smith DM, Chang SC, Park S, Finley D, Cheng Y, Goldberg AL. Docking of the proteasomal ATPases' carboxyl termini in the 20S proteasome's alpha ring opens the gate for substrate entry. Mol Cell. 2007; 27(5): 731-44. (SCI)
  25. Chang SC, Momburg F, Bhutani N, Goldberg AL. The ER aminopeptidase, ERAP1, trims precursors to lengths of MHC class I peptides by a "molecular ruler" mechanism. Proc Natl Acad Sci U S A. 2005; 102(47): 17107-12. (SCI)
  26. Draenert R, Le Gall S, Pfafferott KJ, Leslie AJ, Chetty P, Brander C, Holmes EC, Chang SC, Feeney ME, Addo MM, Ruiz L, Ramduth D, Jeena P, Altfeld M, Thomas S, Tang Y, Verrill CL, Dixon C, Prado JG, Kiepiela P, Martinez-Picado J, Walker BD, Goulder PJ. Immune selection for altered antigen processing leads to cytotoxic T lymphocyte escape in chronic HIV-1 infection. J Exp Med. 2004; 199(7): 905-15. (SCI)
  27. York IA, Chang SC, Saric T, Keys JA, Favreau JM, Goldberg AL, Rock KL. The ER aminopeptidase ERAP1 enhances or limits antigen presentation by trimming epitopes to 8-9 residues. Nat Immunol. 2002; 3(12): 1177-84. (SCI)
  28. Saric T, Chang SC, Hattori A, York IA, Markant S, Rock KL, Tsujimoto M, Goldberg AL. An IFN-gamma-induced aminopeptidase in the ER, ERAP1, trims precursors to MHC class I-presented peptides. Nat Immunol. 2002; 3(12): 1169-76. (SCI)
  29. Chen HM, Chang SC, Wu CC, Cuo TS, Wu JS, Juang RH. Regulation of the catalytic behaviour of L-form starch phosphorylase from sweet potato roots by proteolysis.Physiol Plant. 2002; 114(4): 506-15. (SCI)
  30. Chang SC, Lin PC, Chen HM, Wu JS, Juang RH. The isolation and characterization of Chaperonin 60 from sweet potato roots - Involvement of the chaperonins in starch biosynthesis. Bot Bul Acad Sin. 2000; 41(2): 105-11. (SCI)

其他著作