LI, Hung-Wen
Professor
Room B479, Chemistry Building
Fax: +886-2-3366-8671
Research Interest
- Single-molecule biophysical chemistry.
- DNA homologous recombination mechanism.
- Protein-DNA interactions.
- Optical microscopy tools.
Career and Service Experiences
- B.S., 1993, National Taiwan University.
- Ph.D., 2000, University of California at Berkeley.
- Postdoctoral Research Fellow, 2000-2004, Brandeis University.
- Assistant Professor, 2004-2007, McGill University.
- Associate Professor, 2007-2014, National Taiwan University.
- Professor, 2014-present, National Taiwan University.
Honors and Awards
- Damon Runyon Cancer Research Fund Postdoctoral
Fellowship, 2000-2004.
- Canada FQRNT New Investigator, 2005-2007.Young Investigator Award, Foundation for the Advancement of Outstanding Scholarship, 2007-2012.
- Excellence in Teaching Award, National Taiwan University, 2010-2012.
- Distinguished Teaching Award, National Taiwan University, 2013.
- Outstanding Research Award, Ministry of Science and Technology, 2022.
Selected Publications
- Wei, C.-D., Chang, H.-Y., Lu, C.-H., Chang, C.-C., Furukohri, A., Mwaniki, S., Shinohara, A., Chi, P.* & Li, H.-W.*, "Mei5-Sae3 stabilizes Dmc1 nucleating clusters for efficient Dmc1 assembly on RPA-coated single-stranded DNA", Nucleic Acids Res., 52, 11768 (2024).
- Peng, J.-C., Chang, H.-Y., Sun, Y. L., Prentiss M., Li, H.-W. & Chi, P.*," Hop2-Mnd1 functions as a DNA sequence fidelity switch in Dmc1-mediated DNA recombination", Nature Comm., 15, 9266 (2024).
- Lee, W., Iwasaki, H., Tsubouchi, H.* & Li, H.-W.*, "Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1 filament assembly using distinct mechanisms", Nucleic Acids Res., 51, 8550 (2023).
- Guh, C.L., Lei, K.H., Chen, Y.A., Jiang, Y.Z., Chang, H.Y., Liaw, H., Li, H.-W., Yen, H.Y. & Chi, P*, "RAD51 paralogs synergize with RAD51 to protect reversed forks from cellular nucleases", Nucleic Acids Res., 51, 11717 (2023).
- Lei, K.-H., Yang, H.-L., Chang, H.-Y., Yeh, H.-Y., Nguyen, D. D., Lee, T.-Y., Lyu, X., Chastain, M., Chai, W., Li, H.-W.* & Chi, P.*, "Crosstalk between CST and RPA regulates RAD51 activity during replication stress", Nature Comm., 12, 6412 (2021).
- Lin, Y.-Y., Li, M.-H., Chang, Y.-C., Fu, P.-Y., Ohniwa, R. L., Li, H.-W.* & Lin, J.-J.*, "Dynamic DNA Shortening by Telomere-Binding Protein Cdc13", J. Am. Chem. Soc., 143, 5815 (2021).
- Li, W.-C., Lee, C.-Y., Lan, W.-H., Woo, T.-T., Chen, C.-Y., Yeh, H.-Y., Chang, H.-Y., Liu, H.-C., Chen, C.-L., Chuang, C.-N., Hsueh, Y.-P., Li, H.-W.*, Chi, P.* & Wang, T.-F.*, "Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences", Proc. Natl. Acad. Sci. U.S.A., 118, e2007192118 (2021).
- Luo, S.-C., Yeh, H.-Y., Lan, W.-H., Wu, Y.-M., Yang, C.-H., Chang, H.-Y., Su, G.-C., Lee, C.-Y., Wu, W.-J., Li, H.-W., Ho, M.-C.*, Chi, P.*& Tsai, M.-D.*, "Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures", Nature Comm., 12, 115 (2021).
- Lan, W.-H., Lin, S.-Y., Kao, C.-Y., Chang, W.-H., Yeh, H.-Y., Chang, H.-Y., Chi, P.* & Li, H.-W.* "Rad51 Facilitates Filament Assembly of Meiosis-Specific Dmc1 Recombinase", Proc. Natl. Acad. Sci. U.S.A., 117, 11257 (2020).
- Chang, H.-Y., Lee, C.-Y., Lu, C.-H., Lee, W., Hang, H.-L., Yeh, H.-Y., Li, H.-W.* & Chi, P.* "Microcephaly family protein MCPH1 stabilizes RAD51 filaments", Nucleic Acids Res., 48, 9135 (2020).
- Lin, Y.-H., Chu, C.-C., Wang, P.-Y., Fan, H.-F. Cox, M. M.*, & Li, H.-W.*, "A 5'-to-3' Strand Exchange Polarity is Intrinsic to RecA Nucleoprotein Filaments in the Absence of ATP Hydrolysis", Nucleic Acids Res., 47, 5126 (2019).
- Lu, C.-H., Yeh, H.-Y., Su, G.-C., Ito, K., Kurokawa, Y., Iwasaki, H.*, Chi, P.* & Li, H.-W.* "Swi5-Sfr1 Stimulates Rad51 Recombinase Filament Assembly by Modulating Rad51 Dissociation", Proc. Natl. Acad. Sci. U.S.A., 115, E10059 (2018).
- Li, J.-R., Yu, T.-Y., Chieh, I-.C., Lu, C.-Y., Lin, J.-J.* & Li, H.-W.*, "Pif1 Helicase Regulates Telomerase Activity by Removing from Telomere Ends", Nucleic Acids Res., 42, 8527 (2014).
- Chung, C. & Li, H.-W.*, "Direct Observation of RecBCD Helicase as Single-Stranded DNA Translocases", J. Am. Chem. Soc., 135, 8920 (2013).
Update: 2024-10-29